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. 2017 Mar 2;16(5):812–823. doi: 10.1074/mcp.M116.063719

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — INO80E binds to SUMO1 and SUMO2 through a C-terminal SIM domain. A, Cartoon showing INO80E, the location of the SUMO interaction motif (SIM) at the C-terminus of the protein, and the two SIM-deficient INO80E mutants generated for this study. B, Results from in vitro binding assay. INO80E, the INO80E SIM mutant and the INO80EΔC10 were generated by in vitro translation in the presence of [³⁵S]-methionine and incubated with glutathione sepharose-bound GST-SUMO1 and GST-SUMO2. C, INO80E mtSIM shows reduced binding to GST-SUMO2, but not GST-SUMO1.