Fig. 6.
INO80E selectively binds to SUMO-conjugated TFPT. A, Results from in vitro binding assay of [35S]-methionine-labeled TFPT to wildtype and SIM-deficient INO80E in the presence and absence of E1/E2 enzymes and SUMO. B, Quantitative analysis of these results by scanning densitometry. C, Results from in vivo binding assay of wildtype and mutant TFPT and INO80E the presence and absence of SUMO2. Myc-tagged INO80E was immunoprecipitated, then immunoblotted with anti-V5 to detect coimmunoprecipitated TFPT.