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. 2017 Mar 6;16(5):824–839. doi: 10.1074/mcp.M116.064733

Table II. IGHG AA alleles deduced from mass spectrometry analysis of proteotypic peptides after trypsin digestion of the IG heavy chain. “Observed proteotypic peptides” correspond to a trypsin digestion (cleavage site indicated by a point) of the constant region of the IG gamma chains encoded by a given Homo sapiens IGHG gene; “m/z” is the mass-to-charge ratio corresponding to the peptide, in oxidized form or not, observed with the maximal score; “z” represents the peptide charge state; “Ox” indicates whether the peptide was found at least once in oxidized form (if so, number of occurrences/number of observations) or not (N); “Corresponding IGHG alleles” are mentioned according to amino acid sequences and positions from IMGT repertoire (http://www.imgt.org) and (25); “Attributable IGHG allele combinations” are identified by a slash mark separating the two attributable alleles or groups of alleles.

Purified IgG samples IGHG gene Observed proteotypic peptides m/z z Ox Scoremax Position Corresponding IGHG alleles Attributable IGHG allele combinations
AS50 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 66 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 683.36 4 N 62 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.51 2 N 81 CH2 17–39 all IGHG1
R.EEQYNSTYR.V 595.26 2 N 30 CH2 83–85 all IGHG1
K.TTPPVLDSDGSFFLYSK.La 937.46 2 N 42 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 67 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*06 /
R.EEQFNSTFR.V b 579.26 2 N 31 CH2 83–85 all IGHG2 IGHG2*01*03*04*05*06
R.VVSVLTVVHQDWLNGK.E 897.50 2 N 79 CH2 85.1–101 all IGHG2
K.GFYPSDISVEWESNGQPENNYK.T 1280.57 2 N 54 CH3 26–80 IGHG2*06
IGHG3 R.WQEGNVFSCSVMHEALHNR.F 1122.51 2 5/13 110 CH3 95–116 IGHG3*03 IGHG3*03 /
R.WQEGNIFSCSVMHEALHNR.F 1129.51 2 2/7 111 CH3 95–116 IGHG3*13 IGHG3*13
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 66 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 54 CH2 17–80 all IGHG4 all IGHG4
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 N 55 CH3 95–120 all IGHG4
NP49 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 67 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 68 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.51 2 N 81 CH2 17–39 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 59 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*01*03*04*05*06 /
R.EEQFNSTFR.V b 579.27 2 N 32 CH2 83–85 all IGHG2 all IGHG2
R.VVSVLTVVHQDWLNGK.E 897.50 2 N 83 CH2 85.1–101 all IGHG2
K.TTPPMLDSDGSFFLYSK.Lc 961.45 2 1/4 48 CH3 79–89 all IGHG2
IGHG3 K.GFYPSDIAMEWESSGQPENNYK.T 1275.05 2 1/2 40 CH3 26–80 IGHG3*17*18*19 IGHG3*03 /
R.WQEGNVFSCSVMHEALHNR.F 748.67 3 N 37 CH3 95–116 IGHG3*03 IGHG3*17*18*19
R.WQQGNIFSCSVMHEALHNHYTQK.S 920.09 3 3/8 66 CH3 95–120 IGHG3*17*18*19
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 78 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 71 CH2 17–80 all IGHG4 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 36 CH3 1.1–17 all IGHG4
PA01 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 61 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 64 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.51 2 N 83 CH2 17–39 all IGHG1
K.TTPPVLDSDGSFFLYSK.La 937.46 2 N 60 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 71 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*01*03*04*05*06 /
IGHG2*01*03*04*05*06
IGHG3 R.WQQGNIFSCSVMHEALHNR.F 753.02 3 1/6 76 CH3 95–116 IGHG3*01*02*04 to *12 IGHG3*01*02*04 to *12 /
IGHG3*01*02*04 to *12
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 68 H 3-CH2 13 all IGHG4 IGHG4*01*02*04 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 30 CH2 17–80 all IGHG4 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 48 CH3 1.1–17 all IGHG4
K.TTPPVLDSDGSFFLYSR.L 634.65 3 N 34 CH3 79–89 all IGHG4 except IGHG4*03
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 1/6 52 CH3 95–120 all IGHG4
PA07 IGHG1 K.GPSVFPLAPSSK.S 593.82 2 N 67 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 75 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.50 2 N 88 CH2 17–39 all IGHG1
R.EEQYNSTYR.V 595.26 2 N 31 CH2 83–85 all IGHG1
K.TTPPVLDSDGSFFLYSK.La 937.46 2 N 60 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.95 4 N 86 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*06 /
R.EEQFNSTFR.Vb 579.26 2 N 31 CH2 83–85 all IGHG2 IGHG2*01*03*04*05*06
K.GFYPSDISVEWESNGQPENNYK.T 1280.57 2 N 54 CH3 26–80 IGHG2*06
IGHG3 R.WQQGNIFSCSVMHEALHNR.F 753.02 3 N 105 CH3 95–116 IGHG3*01*02*04 to *12 IGHG3*13 /
R.WQEGNIFSCSVMHEALHNR.F 1129.51 2 2/9 112 CH3 95–116 IGHG3*13 IGHG3*01*02*04 to *12
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 87 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 85 CH2 17–80 all IGHG4 all IGHG4
R.EEQFNSTYR.V 587.26 2 N 31 CH2 83–85 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 46 CH3 1.1–17 all IGHG4
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 2/10 72 CH3 95–120 all IGHG4
PA09 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 67 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 63 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.50 2 N 82 CH2 17–39 all IGHG1
R.EEQYNSTYR.V 595.26 2 N 25 CH2 83–85 all IGHG1
K.TTPPVLDSDGSFFLYSK.La 937.46 2 N 60 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 61 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*06 /
K.GFYPSDISVEWESNGQPENNYK.T 854.05 3 N 41 CH3 26–80 IGHG2*06 IGHG2*01*03*04*05*06
IGHG3 R.WQEGNVFSCSVMHEALHNR.F 1122.51 2 2/8 110 CH3 95–116 IGHG3*03 IGHG3*03 /
R.WQQGNIFSCSVMHEALHNR.F 1129.02 2 1/8 76 CH3 95–116 IGHG3*01*02*04 to *12 IGHG3*01*02*04 to *12
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 65 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 40 CH2 17–80 all IGHG4 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 48 CH3 1.1–17 all IGHG4
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 1/4 61 CH3 95–120 all IGHG4
PA16 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 58 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 62 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.51 2 N 92 CH2 17–39 all IGHG1
K.TTPPVLDSDGSFFLYSK.La 937.46 2 N 51 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 64 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*06 /
K.GFYPSDISVEWESNGQPENNYK.T 1280.57 2 N 53 CH3 26–80 IGHG2*06 IGHG2*01*03*04*05*06
IGHG3 K.GFYPSDIAVEWESSGQPENNYK.T 1259.07 2 N 40 CH3 26–80 IGHG3*06*07*13 IGHG3*03 /
R.WQEGNVFSCSVMHEALHNR.F 1122.51 2 6/15 108 CH3 95–116 IGHG3*03 IGHG3*13
R.WQEGNIFSCSVMHEALHNR.F 753.35 3 N 88 CH3 95–116 IGHG3*13
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 78 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 28 CH2 17–80 all IGHG4 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 42 CH3 1.1–17 all IGHG4
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 N 56 CH3 95–120 all IGHG4
PA31 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 60 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 59 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.51 2 N 81 CH2 17–39 all IGHG1
R.EEQYNSTYR.V 595.26 2 N 30 CH2 83–85 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 65 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*01*03*04*05*06 /
IGHG2*01*03*04*05*06
IGHG3 K.GFYPSDIAMEWESSGQPENNYK.T 1283.05 2 1/1 39 CH3 26–80 IGHG3*17*18*19 IGHG3*03 /
R.WQEGNVFSCSVMHEALHNR.F 1122.51 2 6/12 111 CH3 95–116 IGHG3*03 IGHG3*17*18*19
R.WQQGNIFSCSVMHEALHNHYTQK.S 920.09 3 2/7 62 CH3 95–120 IGHG3*17*18*19
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 74 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 1244.59 3 N 84 CH2 17–80 all IGHG4 all IGHG4
R.EEQFNSTYR.V 587.26 2 N 30 CH2 83–85 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 51 CH3 1.1–17 all IGHG4
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 N 61 CH3 95–120 all IGHG4
PA42 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 64 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 60 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.50 2 N 84 CH2 17–39 all IGHG1
R.EEQYNSTYR.V 595.26 2 N 30 CH2 83–85 all IGHG1
K.TTPPVLDSDGSFFLYSK.L 937.46 2 N 64 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 74 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*06 /
K.GFYPSDISVEWESNGQPENNYK.T 1280.57 2 N 53 CH3 26–80 IGHG2*06 IGHG2*01*03*04*05*06
IGHG3 K.TPLGDTTHTCPR.C 649.81 2 N 75 H1 3–16 all IGHG3 IGHG3*01*02*04 to *12 /
K.SCDTPPPCPR.C 536.73 2 N 45 H2 H3 H4 3–14 all IGHG3 all IGHG3
R.TPEVTCVVVDVSHEDPEVQFK.W 1179.02 2 N 81 CH2 17–41 all IGHG3
R.WQQGNIFSCSVMHEALHNR.F 753.02 3 3/13 124 CH3 95–116 IGHG3*01*02*04 to *12
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 73 H 3-CH2 13 all IGHG4 IGHG4*01*02*04 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 933.69 4 N 76 CH2 17–80 all IGHG4 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 53 CH3 1.1–17 all IGHG4
K.TTPPVLDSDGSFFLYSR.L 951.47 2 N 46 CH3 79–89 all IGHG4 except IGHG4*03
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 3/9 70 CH3 95–120 all IGHG4
PA45 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 64 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 64 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.51 2 N 89 CH2 17–39 all IGHG1
R.EEQYNSTYR.V 595.26 2 N 40 CH2 83–85 all IGHG1
R.VVSVLTVLHQDWLNGK.E d 904.50 2 N 68 CH2 85.1–101 all IGHG1
K.TTPPVLDSDGSFFLYSK.L 937.46 2 N 60 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 935.94 4 N 62 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*01*03*04*05*06 /
IGHG2*01*03*04*05*06
IGHG3 K.TPLGDTTHTCPR.C 649.81 2 N 81 H1 3–16 all IGHG3 IGHG3*01*02*04 to *12 /
K.SCDTPPPCPR.C 536.73 2 N 45 H2 H3 H4 3–14 all IGHG3 all IGHG3
R.TPEVTCVVVDVSHEDPEVQFK.W 1179.07 2 N 75 CH2 17–41 all IGHG3
R.WQQGNIFSCSVMHEALHNR.F 1129.03 2 2/8 82 CH3 95–116 IGHG3*01*02*04 to *12
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 51 H 3-CH2 13 all IGHG4 IGHG4*01*02*04 /
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 50 CH3 1.1–17 all IGHG4 all IGHG4
K.TTPPVLDSDGSFFLYSR.L 951.47 2 N 60 CH3 79–89 all IGHG4 except IGHG4*03
R.WQEGNVFSCSVMHEALHNHYTQK.S 915.75 3 N 52 CH3 95–120 all IGHG4
PA48 IGHG1 K.GPSVFPLAPSSK.S 593.83 2 N 67 CH1 1.1–13 all IGHG1 all IGHG1 /
K.THTCPPCPAPELLGGPSVFLFPPKPK.D 910.81 3 N 63 H 7-CH2 13 all IGHG1 all IGHG1
R.TPEVTCVVVDVSHEDPEVK.F 1041.50 2 N 88 CH2 17–39 all IGHG1
K.TTPPVLDSDGSFFLYSK.La 937.46 2 N 45 CH3 79–89 all IGHG1
IGHG2 R.TPEVTCVVVDVSHEDPEVQFNWYVDGVEVHNAK.T 1247.59 3 N 72 CH2 17–80 all IGHG2 except IGHG2*02 IGHG2*01*03*04*05*06 /
R.EEQFNSTFR.V b 579.27 2 N 25 CH2 83–85 all IGHG2 all IGHG2
R.VVSVLTVVHQDWLNGK.E 897.50 2 N 75 CH2 85.1–101 all IGHG2
K.TTPPMLDSDGSFFLYSK.Lc 953.45 2 1/4 50 CH3 79–89 all IGHG2
IGHG3 K.TPLGDTTHTCPR.C 649.81 2 N 94 H1 3–16 all IGHG3 IGHG3*03 /
K.SCDTPPPCPR.C 536.73 2 N 45 H2 H3 H4 3–14 all IGHG3 all IGHG3
R.TPEVTCVVVDVSHEDPEVQFK.W 1179.07 2 N 81 CH2 17–41 all IGHG3
R.WQEGNVFSCSVMHEALHNR.F 1122.50 2 4/11 110 CH3 95–116 IGHG3*03
IGHG4 K.YGPPCPSCPAPEFLGGPSVFLFPPKPK.D 943.81 3 N 64 H 3-CH2 13 all IGHG4 all IGHG4 /
R.TPEVTCVVVDVSQEDPEVQFNWYVDGVEVHNAK.T 933.69 4 N 66 CH2 17–80 all IGHG4 all IGHG4
R.EPQVYTLPPSQEEMTK.N 938.95 2 N 53 CH3 1.1–17 all IGHG4
R.WQEGNVFSCSVMHEALHNHYTQK.S 1373.12 2 N 59 CH3 95–120 all IGHG4

a peptide CH3 79–89 attributed to all IGHG1 alleles and not to IGHG4*03 also proposed in subsets (not considered in the deduction strategy).

b peptide CH2 83–85 attributed to all IGHG2 alleles and not to IGHG3*11 and IGHG3*12 also proposed in subsets (not considered in the deduction strategy).

c peptide CH3 79–89 attributed to IGHG2*01*03*04*05*06 group of alleles and not to IGHG3*06, IGHG3*07, IGHG3*13 or IGHG3*15 alleles proposed in subsets (not considered in the deduction strategy).

d peptide CH2 85.1–101 attributed to all IGHG1 alleles and not to IGHG4 (all alleles except IGHG4*02) or IGHG3 (all alleles except IGHG3*09) proposed in subsets (not considered in the deduction strategy).