Table V. IGHG4 AA alleles deduced from genomic sequencing of the CH2 and CH3-CHS exons. As IGHG4 is encoded on chromosome 14 (14q 32.3), two IGHG4 alleles are expressed per sample. Amino acid changes not referenced in IMGT® are mentioned in bold.
| DNA samples | Nt substitutions and AA changes with reference to IGHG4*01 (K01316) |
Genomic IMGT IGHG4 alleles determined | IGHG4 AA alleles deduced | ||
|---|---|---|---|---|---|
| Nt IMGTa | AA IMGTa | AA EUb | |||
| AS50 | CH3 a267>c | CH3 L89 | CH3 L410 = | IGHG4*04 | IGHG4*04 |
| CH2 g271>a, a322>c; CH3 a32>g, g243>a, a267>c | CH2 V91>I, N108>H; CH3 Q11>R, T81, L89 | CH2 V308I, N325H; CH3 Q355R, T394 =, L410 = | not referenced | IGHG4*[V91>I;N108>H;Q11>R] | |
| NP49 | CH3 a267>c | CH3 L89 | CH3 L410 = | IGHG4*04 | IGHG4*04 |
| CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 | |
| PA01 | CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 |
| CH2 g3.4>a, t42>c | CH2 E1.4, T14 | CH2 E233 =, T250 = | not referenced | IGHG4*01*04 | |
| PA07 | CH2 g271>a, a322>c; CH3 a267>c | CH2 V91>I, N108>H; CH3 L89 | CH2 V308I, N325H; CH3 L410 = | not referenced | IGHG4*[V91>I;N108>H] |
| CH2 g3.4>a, t42>c | CH2 E1.4, T14 | CH2 E233 =, T250 = | not referenced | IGHG4*01*04 | |
| PA09 | CH3 a267>c | CH3 L89 | CH3 L410 = | IGHG4*04 | IGHG4*04 |
| CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 | |
| PA16 | CH2 g271>a, a322>c; CH3 a32>g, g243>a, a267>c | CH2 V91>I, N108>H; CH3 Q11>R, T81, L89 | CH2 V308I, N325H; CH3 Q355R, T394 =, L410 = | not referenced | IGHG4*[V91>I;N108>H;Q11>R] |
| CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 | |
| PA31 | CH3 a267>c | CH3 L89 | CH3 L410 = | IGHG4*04 | IGHG4*04 |
| CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 | |
| PA42 | - | - | - | IGHG4*01 | IGHG4*01 |
| CH3 a267>c | CH3 L89 | CH3 L410 = | IGHG4*04 | IGHG4*04 | |
| PA45 | - | - | - | IGHG4*01 | IGHG4*01 |
| - | - | - | IGHG4*01 | IGHG4*01 | |
| PA48 | CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 |
| CH2 g3.4>a | CH2 E1.4 | CH2 E233 = | not referenced | IGHG4*01*04 | |
a Nomenclature according to the IMGT unique numbering for C-DOMAIN (2, 25) and IMGT Allele alignment Homo sapiens IGHG4 (http://www.imgt.org/IMGTrepertoire/Proteins/alleles/index.php?species=Homo%20sapiens&group=IGHC&gene=IGHG4).
b Nomenclature adapted from Human Genome Variation Society (www.hgvs.org) and (29); amino acid (AA) EU numbering according to IMGT® www.imgt.org (23).