Table VI. Confrontation of serological, proteomic and genomic approaches for IGHG3 allele deduction or determination. As IGHG3 is encoded on chromosome 14 (14q 32.3), two IGHG3 alleles are expressed for each sample.
| Samplesa | Combination of IGHG3 alleles according to the method |
Discordances between methodsb |
||||
|---|---|---|---|---|---|---|
| Serology (Ser) | Proteomics (Pro) | Genomics (Gen) | Ser vs. Pro | Ser vs. Gen | Pro vs. Gen | |
| AS50 | IGHG3*01*05*06*07*09*10*11*12 | IGHG3*03 | IGHG3*03 | X | X | |
| IGHG3*01*05*06*07*09*10*11*12 | IGHG3*13 | IGHG3*13 | X | X | ||
| NP49 | IGHG3*17 | IGHG3*03 | IGHG3*03 | X | X | |
| IGHG3*17 | IGHG3*17*18*19 | IGHG3*01*04*05*10 | X | X | ||
| PA01 | IGHG3*17 | IGHG3*01*02*04 to *12 | IGHG3*01*04*05*10 | X | X | |
| IGHG3*01*05*06*07*09*10*11*12 | IGHG3*01*02*04 to *12 | IGHG3*01*04*05*10 | ||||
| PA07 | IGHG3*13 | IGHG3*13 | IGHG3*13 | |||
| IGHG3*01*05*06*07*09*10*11*12 | IGHG3*01*02*04 to *12 | IGHG3*01*04*05*10 | ||||
| PA09 | IGHG3*03 | IGHG3*03 | IGHG3*03 | |||
| IGHG3*01*05*06*07*09*10*11*12 | IGHG3*01*02*04 to *12 | IGHG3*01*04*05*10 | ||||
| PA16 | IGHG3*13 | IGHG3*03 | IGHG3*03 | X | X | |
| IGHG3*13 | IGHG3*13 | IGHG3*13 | ||||
| PA31 | IGHG3*03 | IGHG3*03 | IGHG3*03 | |||
| IGHG3*17 | IGHG3*17*18*19 | IGHG3*17 | ||||
| PA42 | IGHG3*13 | IGHG3*01*02*04 to *12 | IGHG3*01*04*05*10 | X | X | |
| IGHG3*17 | all IGHG3 | IGHG3*01*04*05*10 | X | |||
| PA45 | IGHG3*01*05*06*07*09*10*11*12 | IGHG3*01*02*04 to *12 | IGHG3*01*04*05*10 | |||
| IGHG3*01*05*06*07*09*10*11*12 | all IGHG3 | IGHG3*01*04*05*10 | ||||
| PA48 | IGHG3*03 | IGHG3*03 | IGHG3*03 | |||
| IGHG3*03 | all IGHG3 | IGHG3*03 | ||||
a Individual samples consisted in plasma (serology), purified IgG (proteomics) or genomic DNA (genomics).
b Discordances between methods are mentioned by “X”.