Fig. 5.

Validation of iTRAQ data in human tissue. Expression changes of five proteins (S100A6, lumican, plastin-2, 14-3-3 zeta/delta and vimentin) were evaluated by Western blot and immunohistochemical staining of human tissue. A, Western blot analysis in seven paired tumor cases (N, adjacent nontumor and T, tumor tissue); B, the relative band intensities were normalized by average normal and plotted as a scatter plots. Asterisk (*) denotes significant difference (p < 0.05) by Student's t test versus “Normal” group and p values are shown on the bar plots; C, Immunohistochemical staining of the five proteins in the same human tumor tissue samples showed only minor staining in nontumor tissue (original magnification, ×200).