Fig. 3.

Differences in the ADP-ribosylation level in ovarian cancer cells at basal and H₂O₂-treated conditions. A, The heatmap shows the significantly regulated ADP-ribosylated proteins in oxidative stress in two different ovarian cancer cell lines (A2780 and SKOV3) (n = 2, ANOVA, p < 0.05), detected using PRM MS measurement. The values were log2 transformed. B, The ADP-ribosylation level of H2B histone variants for SKOV3 and A2780 detected at basal/untreated condition detected using PRM MS measurement. The values were log2 transformed (n = 2). C, Examples of oxidative stress-induced ADP-ribosylated targets for A2780 and SKOV3 at different concentrations of H₂O₂ detected using PRM MS measurement. The values were log2 transformed and plotted separately, the dashed lines connect the average values (n = 2). D, The level of ARTD1 in A2780 and SKOV3 cells (n = 6, mean ± S.D., student t test, p = 0.000083), detected using PRM MS measurement.