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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Neurol Clin. 2017 May;35(2):175–190. doi: 10.1016/j.ncl.2017.01.001

Fig. 3.

Fig. 3

Amnis Imagestream can be used to examine astrocyte phagocytosis of human synap- tosomes (A–D). Synaptosomes were labeled with pHrodoRed (Lifetech) and incubated with iPS-derived astrocytes. Astrocytes are then labeled with CD44 and MERTK and cells that were CD44-positive were examined for the presence of pHrodoRed. (A) Flow cytometry analysis of astrocytes to gate for phagocytic events. (B) Representative images of iPS-derived astrocytes can evaluate viability, and can be used to examine colocalization between synaptosome- containing cellular compartments and MERTK. (C) iPS astrocytes can functionally phagocy- tose synaptosomes to the same extent as fetal astrocytes. (D) Essentially all iPS-derived astrocytes that demonstrate a phagocytic event express MERTK, which has been shown to be essential for astrocyte-mediated synaptic pruning.