Figure 2.

Affinity-activity relation of IFN-1 signaling. A, antiviral (AV) and antiproliferative (AP) response of WISH cells to IFN-1 treatment. Each dot represents the mean values of six independent antiviral and antiproliferative experiments (5). For clarity, the fraction of antiviral response (y axis) is shown as relative light transmission, while the fraction of antiproliferative response is shown as absorbance upon crystal violet staining (thus antiviral cell survival increases with IFN-1, whereas cell numbers decrease with increased antiproliferative response). B, relative to WT IFNα2, biological potency (EC₅₀, antiviral, antiproliferative) is plotted against interferon receptor-binding affinity of IFNα2 mutants as a measure using surface plasmon resonance.