RNF11 is enriched in the crude endosomal fraction.
A, schema of the procedure used to prepare samples for mass spectrometry. B, RNF11 is enriched in a HEK293T membrane fraction obtained after subcellular fractionation. 25 μg of protein were loaded per lane. Immunoblots (WB) were probed with the in-house RNF11 antibody, anti-β-tubulin antibody, anti-Lamp1 antibody (lysosomal marker), and anti-Rab7 antibody (lysosomal marker). The HEK293T P20K and P100K fractions are defined under “Experimental procedures.”