Figure 10.

Sequence alignment of Cpin_6279 and its homologs. Multiple sequence alignment was performed with Clustal Omega (55). Identical amino acids are highlighted in red, and conservative residues are boxed. Helices (α), strands (β), and 3₁₀ helices (η) are denoted above the alignment. Letters in parentheses correspond to the secondary structural elements shown in Fig. 9A. The residues corresponding to highly conserved acidic amino acids in the active site are indicated by solid stars. The GenBank^TM accession numbers of the proteins used for the alignment are as follows: Cpin_6279 (ACU63684.1); BF9343_0330 (CAH06109.1); BACCAC_03554 (EDM19439.1); BACUNI_03963 (EDO52350.1); BT_3566 (AAO78672.1); Bovatus_03682 (ALJ48288.1); Fjoh_3523 (ABQ06537.1); and Dfer_4452 (ACT95653.1). The figure was prepared with ESPript (56).