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. 2017 Mar 17;292(18):7519–7530. doi: 10.1074/jbc.M116.773960

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Figure 1. — GR interacts with the transactivation domains of NRF2. A, list of NRF2-interacting proteins identified by LC-MS/MS from Keap1^f/f::albumin-Cre mouse livers. PSM, total peptide spectrum matches. B, immunoblotting analysis of GR in the NRF2 complex. C, constructs of GST-fusion proteins of NRF2 mutants. 1, GST; 2, GST-Neh2(1–84); 3, GST-Neh4/5(85–210); 4, GST-Neh6u(211–407); 5, GST-Neh6d(319–408); 6, GST-Neh1(408–554); 7, GST-Neh3(555–598). D, FLAG-GR overexpressed in 293T cells, which was used for the interaction study with GST-fusion NRF2 mutants. E, GST-NRF2 pulldown assay with FLAG-GR. FLAG-GR was purified from FLAG-GR-expressing 293T cells with an anti-FLAG antibody and incubated with GST-NRF2 mutants. FLAG-GR was detected using an anti-FLAG antibody (top). A protein staining of GST-NRF2 mutants is shown (bottom). CBB, Coomassie Brilliant Blue.