GR represses inducible expression of NRF2 target genes in response to DEM.
A, relative expression levels of NRF2 target genes in Hepa1c1c7 cells treated with DEM, DEM + Dex, and DEM + Bet. B, protein levels of GR in Hepa1c1c7 cells with or without GR knockdown. C, relative expression levels of NRF2 target genes in Hepa1c1c7 cells with or without GR knockdown. Cells were treated with DEM, DEM + Dex, and DEM + Bet. -Fold changes were calculated against vehicle-treated samples, and the average and S.D. (error bars) of the -fold changes from three independent experiments are shown (A and C). Student's t test (A) and one-way ANOVA and Tukey's post hoc test (C) were performed as statistical analyses. *, p < 0.05; **, p < 0.01; ns, nonsignificant.