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. 2017 Mar 13;292(18):7554–7565. doi: 10.1074/jbc.M116.773788

Figure 5.

Figure 5.

IFN-β FLETVR variant induces reduced STAT1 phosphorylation compared with IFN-β. A, L929 cells were treated with either 1 ng/ml or 5 ng/ml IFN-β or the IFN-β FLETVR variant for either 30 or 120 min. STAT1 phosphorylated at Tyr701, total STAT1, and actin were detected in whole cell lysates. This result is representative of triplicate independent experiments. B, densitometry of Western blots; data from the triplicate independent experiments are represented as intensity of phospho-STAT1 relative to intensity of actin. Data are expressed as the mean ± S.D. of triplicate independent experiments. Significance relative to untreated samples. *, p < 0.05; **, p < 0.01; ****, p < 0.0001 (two-way ANOVA with Tukey's multiple comparisons testing); significance relative to treatment with 1 ng/ml IFN-β for 30 min. #, p < 0.05; ###, p < 0.001; ####, p < 0.0001 (2-way ANOVA with Tukey's multiple comparisons testing).