Figure 3.

TrkC-miR2 direct interaction with its predicted target gene. A, RT-qPCR result shows APC2 gene down-regulation after TrkC-premir2 overexpression in the SW480 cell line compared with the related controls. B, dual luciferase assay supported TrkC-miR2 direct interaction with 3′-UTR sequence of APC2 target gene. C, lack of interaction between the overexpressed TrkC-miR2 and mutated APC2,3′-UTR detected by dual luciferase assay and supported direct interaction between this miRNA and the wild-type sequence of APC2,3′-UTR sequence shown in B. *, p < 0.05.