Table 1.
Primer and oligo sequences that were used in the study
| Primer name | Primer sequence, 5′ to 3′ | Amplicon size (base pairs) |
|---|---|---|
| TrkC-real time | Forward, CCTGTGTCCTGTTGGTGGTTCTC | 195 |
| Reverse, GAGTCATGCCAATGACCACAGTGTC | ||
| TrkC-premir2 | GATTCTGAGCTGCACAGTCCAG | |
| TrkC-miR2-5p | GGCTGGGGATTCTGAGCT | |
| U48 | Forward, TGACCCCAGGTAACTCTGAGTGTGT | |
| Anchored oligo (dT) | GCGTCGACTAGTACAACTCAAGGTTCTTCCAGTCACGACG (T)18V | |
| Universal-outer | GCGTCGACTAGTACAACTCAAG | |
| Universal-inner | AACTCAAGGTTCTTCCAGTCACG | |
| B-cat-real time | Forward, AGAACAGAGCCAATGGCTTG | 130 |
| Reverse, CCTGGCCATATCCACCAGAG | ||
| c-myc-real time | Forward, CTCCTACGTTGCGGTCACAC | 142 |
| Reverse, CGGGTCGCAGATGAAACTCT | ||
| GAPDH | Forward, GCCACATCGCTCAGACAC | 115 |
| Reverse, GGCAACAATATCCACTTTACCAG | ||
| TrkC-Intron | Int-F: CTGGCGGCCGCTGAACAAGGGAGATGGCTCAGTGG | 802 |
| Int-R: TAGACGCGTGGCTTTGCTGTCACCGCTGAGG | ||
| APC2-3′-UTR | Forward, GGGCGAAGCCTGTAATCACTGC | 2515 |
| Reverse, GAGTCGGACAGCTGACGGTG | ||
| Axin1-3′-UTR | Forward, AAGGTGGACTGATAGGCTGGT | 715 |
| Reverse, AGAAGACACACCACAGCCAGG | ||
| APC1-3′-UTR | Forward, TGGAACCCAAAGTCCTAAGC | 2140 |
| Reverse, CTGGGAAAACAACAGAAGTAG | ||
| APC2-real time | Forward, TCCCAGCTCCCTGCCTCTGT | 129 |
| Reverse, AGCCAGCCAGACCCAAGTTCT | ||
| APC1-real time | Forward, TATTACGGAATGTGTCCAGCTTG | 133 |
| Reverse, CCACATGCATTACTGACTATTGTC | ||
| Axin1-real time | Forward, ATGCAGGAGAGCGTGCAGGTC | 237 |
| Reverse, TGACGATGGATCGCCGTCCTC |