Figure 1.
ACBP stimulates CerS2 and CerS3 activity in vitro. Homogenates were prepared from HEK293T cells overexpressing Cers1 (A), CerS2 (B), CerS3 (C), CerS4 (D), CerS5 (E), or CerS6 (F) or a catalytic inactive form of CerS3 (W15R) (G). CerS activity was determined using C18:0-CoA (A), C24:1-CoA (B), C26:0-CoA (C, G, and H), C20:0-CoA (D), and C16:0-CoA (E and F), respectively, in the presence of increasing amounts of ACBP. H, CerS3 activity was assayed in the presence of increasing amounts of C26:0-CoA in the presence or absence of 1 nm recombinant ACBP. The results are mean values ± S.D., n = 3 for A–G, and n = 6 for H.