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. 2017 Mar 19;292(18):7588–7597. doi: 10.1074/jbc.M117.785345

Figure 3.

Figure 3.

Co-expression of ACBP in HEK293T cells increases CerS2 and CerS3 activity. CerS2-HA (A) or CerS3-HA (B) were co-expressed in HEK293T cells with Flag-tagged ACBP, and CerS2 or CerS3 activity was assayed. CerS2 or CerS3 activity was also assayed in cell lysates from cells transfected with CerS2 or CerS3 after the addition of purified, recombinant ACBP. The data shown represent means ± S.D., n = 3. Statistical analyses were performed using unpaired t test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.