Table 2.

Experimental and computed PELDOR interspin distances for the labels in the His-kinase region

Parallel and antiparallel structures of a homology model of the Agp1 histidine kinase that was predicted based on a T. maritima histidine kinase (12). Half-widths at the half-maxima are given in parentheses.

His-kinase spin-label positions	Computed distance (parallel subunits)	Experimental distance
	nm	nm
K517R1-K517R1	2.3 (0.4)	2.7 (0.3)
H528R1-H528R1	2.7 (0.3)	3.1 (0.4)
R535R1-R535R1	2.7 (0.3)	2.7 (0.4)
K554R1-K554R1	2.3 (0.4)	3.2 (0.1)
R603R1-R603R1 (inactive)	6.0 (0.7)	2.6 (0.4)
R603R1-R603R1 (active)	3.1 (0.15)	2.6 (0.4)