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Raw isotherms of heparin and heparan sulfate ITC titrations into AgRP peptides. Size-heterogeneous heparin and heparan sulfate were titrated into AgRP variants as indicated. A–C, AgRP(83–132), AgRP(83–120), and AgRP4K isotherms are shown after titration with heparin (left panels) and heparan sulfate (right panels). D, AgRP(87–120), AgRP(87–132), and AgRP4Q isotherms after titration with heparin. As shown, these peptides do not bind heparin and also do not bind heparan sulfate.
