Figure 5.

ITC results with AgRP variants. Each AgRP variant was titrated with either size-heterogeneous heparin or heparan sulfate. Thermodynamic data were measured, including dissociation constants and stoichiometry. GAG binding appears to be directly correlated to the non-ICK positive charge. As shown in the % Feeding Above Saline column, taken from our previous rat feeding study, this trend correlates with 24-h feeding. Four ITC experiments were repeated with each peptide two to four times, and the reported error is the standard deviation of each set of measurements. Feeding data are presented as percent feeding above saline and standard deviation.