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. 2017 Mar 6;292(18):7651–7661. doi: 10.1074/jbc.M116.772822

Figure 6.

Figure 6.

HSQC chemical shift perturbation data from titration of heparin into AgRP peptides. A, top panels, HSQC data of AgRP(83–132) titrated with heparin dp6 showing residues from both C-terminal loop (Lys-121, Leu-122, and Ala-125) and N-terminal segment (Arg-86) shifting. A and B, bottom panels, data plotted as Δ chemical shift. A Δδ value is considered significant when it exceeds 1 S.D. over the mean chemical shift. Non-ICK regions, which are important for GAG binding by ITC, are boxed. B, top panel, HSQC data of AgRP4K titrated with heparin dp2. Residues from both the C-terminal loop (Lys-121, Leu-122, Lys-123, and Lys-125) and N-terminal segment (Arg-85 and Arg-86) shift systematically with added dp2. Non-ICK regions are boxed. We were unable to observe Lys-83 and Lys-84 because of solvent exchange.