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. 2017 Mar 3;292(18):7662–7674. doi: 10.1074/jbc.M117.775114

Figure 3.

Figure 3.

Sensitivity of SCE1 mutants to egress agonists. Shown is egress of wild-type, Δsce1, Δcdpk3, and Δcdpk3Δsce1 parasites stimulated with either A23187 or BIPPO. A, egress assay measured at 2.5, 5, and 15 min. There was no difference in egress capacity between the wild type and Δsce1 at any time point. B, egress at 5 min over a concentration gradient of A23187. The only statistically significant differences observed are by comparison between all strains and with Δcdpk3 for the two highest concentrations of A23187. C, egress in response to BIPPO, measured at 2.5, 5, and 15 min. There was no statistically significant difference between the wild type and Δsce1 at any time point. D, egress in response to BIPPO at 5 min over a concentration gradient. The only statistically significant differences observed are by comparison between all strains and Δcdpk3 for the two highest concentrations of A23187. Error bars show mean ± S.D. All statistical tests were performed using two-way ANOVA and Tukey correction for multiple comparisons. The data were derived from three independent biological replicates.