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. Author manuscript; available in PMC: 2018 Mar 4.
Published in final edited form as: Oncogene. 2016 Nov 7;36(18):2589–2598. doi: 10.1038/onc.2016.416

Figure 4.

Figure 4

PAR-1 promotes the adhesive properties and bone marrow homing/engraftment of MLL-AF9 cells. (a) Wild-type and PAR-1-deficient MLL-AF9 colony-forming cells were seeded into Retronectin-precoated wells with 0, 0.5 or 1 U/ml thrombin. The number of adherent cells was estimated using WST-1 after the removal of non-adherent cells. Data are shown as mean ± s.d. from two independent experiments. Note that the addition of thrombin significantly increased cell adhesion in PAR-1-expressing MLL-AF9 cells, but not in PAR-1-deficient cells. (b) Engraftment of MLL-AF9-expressing wild-type or PAR-1-deficient colony-forming cells (CD45.2+GFP+ cells) at 16 or 96 h post-transplant in the bone marrow and spleen of recipient mice. PAR-1 deficiency significantly limited bone marrow engraftment 96 h post-transplant. Data are show as mean ± s.d. N = 6 for each group.