FIG.1.

A mutation in a splice site of otogelin underlies rock solo ^AN66 phenotype. a Top: exon structure of otog and location of the splice site mutation in rock solo ^AN66 (first asterisk) and the early termination mutation in the otog ^sa10228 line (second asterisk). Bottom: electropherograms of otog sequencing in the rock solo ^AN66 (left) and otog ^sa10228 (right) lines at the loci identified. Data are from pooled larvae. b Transmitted light images of the ear, viewed from the lateral aspect, in 5–7 dpf larvae. The anterior (utricular) otolith (dashed outline) is absent in both the rock solo ^AN66 homozygotes as well as in offspring from the complementation cross between rock solo ^AN66 and otog ^sa10228. Rostral is to the left and dorsal is up.