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. 2017 Mar 3;16(3):508–517. doi: 10.1111/acel.12576

Figure 4.

Figure 4

Srebp‐1c was required for CR‐associated activation of mitochondrial biogenesis in WAT. (A, B) mRNA expression levels of mitochondrial‐related genes, Pgc‐1α (A) and Cox4 (B), in WAT from four groups of fed mice were analyzed by real‐time RT–PCR. Data were normalized against values for Tbp expression (n = 3–5). (C) Ratio of mitochondrial (COX2) vs. nuclear (β‐globin) DNA in WAT from four groups of fed mice was obtained by real‐time PCR (n = 4–6). (D) Citrate synthase (CS) activity in WAT from four groups of fed mice was measured spectrophotometrically at 412 nm. Values in all panels are means ± SEM. *P < 0.05, **P < 0.01 vs. AL, †P < 0.05 and ††P < 0.01 vs. WT, analyzed by Tukey's test.