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. 2017 May 5;10:126. doi: 10.3389/fnmol.2017.00126

FIGURE 6.

FIGURE 6

miR-30b agomir down regulated Nav1.3 and alleviated neuropathic pain. (A,C) Ipsilateral paw withdrawal to mechanical and thermal thresholds, ∗∗∗P < 0.001 vs. naïve + scramble; #P = 0.025, ##P = 0.0062, ###P = 0.00048; #P = 0.032, ###P = 0.00053, ###P = 0.00025 vs. SNL + scramble, two-way ANOVA, n = 6 rats. (B,D) Contralateral paw withdrawal to mechanical and thermal thresholds, P > 0.05 vs. naïve + scramble; P > 0.05 vs. SNL + scramble, two-way ANOVA, n = 6 rats. (E,F) The relative expression of miR-30b (E) and SCN3A (F) with intrathecal miR-30b agomir in DRG neurons of SNL rats was determined by qRT-PCR, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. naïve + scramble; ##P = 0.0034, ##P = 0.0018 vs. SNL + scramble, one-way ANOVA, n = 3 rats. (G) Nav1.3 protein expression in DRG neurons of SNL rats at 14 days injected with intrathecal miR-30b agomir, ∗P = 0.0303 vs. SNL + scramble, two-tailed paired t-test, n = 3 rats. (H,I) The relative expression of miR-30b (H) and SCN3A (I) with intrathecal miR-30b agomir in spinal cord of SNL rats, ∗P < 0.05, ∗∗P < 0.01 ∗∗∗P < 0.001 vs. naïve + scramble; #P = 0.0366, ###P = 0.00087 vs. SNL + scramble, one-way ANOVA, n = 3 rats. (J) Nav1.3 protein expression in spinal cord of SNL rats at 14 days injected with intrathecal miR-30b agomir, ∗P = 0.0110 vs. SNL + scramble, two-tailed paired t-test, n = 3 rats. Data are shown as means ± SEM.