Fig. 5.

ACF carpet analysis of intranucelar diffusion in an interphase nucleus. a A line is scanned across the chromatin in the interphase nucleus (scale bar 5 μm). b– e Free GFP (green) in a CHOK1 nucleus stained with Hoechst 33342 (blue). f, g Intensity profiles of GFP and Hoechst 33342 across the line drawn. h, i Fluorescence intensity carpet and ACF carpet of the line drawn across freely diffusing GFP. Figure partly taken from Hinde et al. (2010)