Differential sedimentation coefficient distribution of anti-IgE, FcεRIα, IgE:anti-IgE, and IgE:FcεRIα complexes (a) and anti-IgE2, FcεRIα, IgE:anti-IgE2, and IgE:FcεRIα complexes (b). Assessment of competition of binding of soluble high affinity receptor, FcεRIα, with either preformed IgE:anti-IgE or IgE:anti-IgE2 complexes using AUC sedimentation velocity