Fig. 4.

Viscosity of MAb as a function of protein concentration without any added salt. Non-lyophilized samples prepared by TFF with a composition of 266 mM sucrose and 16 mM histidine at pH 6.0: MAb 1 (♦), MAb 2 () and MAb 3 (). Lyophilized and reconstituted samples: MAb 1 lyophilized and reconstituted (●) and contains 266 mM sucrose, 16 mM histidine, and 0.03 % polysorbate 20 at pH 6; MAb 2 (■) Lyophilized and reconstituted samples are at either 100 mg/mL MAb 2 in 240 mM trehalose, 40 mM histidine, 0.04 % polysorbate 20 or 125 mg/mL in 300 mM trehalose, 50 mM histidine, and 0.05 % polysorbate 20. The solid curves are the result of a non-linear regression of the data to Eq. 1 using a solvent viscosity of 1.1 mPas and intrinsic viscosity of 6.3 cm3/g for an IgG1