Table 2.
Parameters in the transport coefficient, kt
| Parameters | Biacore-specific data | |
|---|---|---|
| α | Distance from the flow cell inlet to the center of the detection spot (m) | 1.28*10−3 |
| β | Detection spot length (m) | 1.80*10−3 |
| D | Diffusion coefficient (m2·s) | |
| F | Volumetric flow rate (m3·s−1) | |
| h | Flow cell height (m) | 4*10−5 |
| Mw | Molecular weight (g·mole−1) | |
| w | Flow cell width (m) | 5.00*10−4 |
Note that Biacore-specific data vary for different instruments and flow cell designs. The values provided here are relevant for the Biacore T200 flow cell FC 2–1 configuration