Fig. 2.
Lysylendopeptidase digestion of the wedge intermediates. a (gp10)3 before (lane 1) and after (lane 2) digestion. b (gp7)(gp10)3 before (lane 1) and after (lane 2) digestion. In the presence of gp7, gp10 becomes protease resistant and binds the 14-kDa C-terminal fragment of gp7 (gp7C residues 908–1032). c (gp7)(gp10)3(gp8)2 before (lane 1) and after (lane 2) digestion; lane 3 Size Exclusion Chromatography purified digested product. In the presence of gp8, the associated C-terminal fragment of gp7 to gp10 becomes larger to be 18 kDa (gp7C residues 877–1032). d (gp10)3(gp11)3 before (lane 1) and after (lane 2) digestion. In the presence of gp11, gp10 becomes partially protease resistant and gives larger C-terminal fragment (gp10C residues 196–602). The rationale of this difference is now explained based on the quaternary structure of the complex and discussed in the text. Lane M Molecular weight marker. In a and d, the molecular weights are indicated in the figure; in b, the molecular weights are the same as in a; in c, the molecular weights are 200, 116, 97, 66, 45, 31, 21, 14 and 6 kDa, respectively, beginning from the top of the gel