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. 2017 May 3;8:14959. doi: 10.1038/ncomms14959

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Copyright © 2017, The Author(s)

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(a) Reactions used a 5′-³²P (*) 20 bp duplex or a 1-nt gapped DNA (10 nM) incubated with hEXOG_(monomer) at 7, 14, 28, 70 and 140 nM for 5 min. Products were visualized after denaturing polyacrylamide gel electrophoresis. (b) Quantification of product from reactions in a. (c) Time-dependent exonuclease reaction using 200 nM 3′-F-labelled 20 bp duplex with 66 nM hEXOG_(monomer). (d) Time-dependent product formation at various hEXOG_(monomer) concentrations. Control reactions: H140A hEXOG (o) and wild-type enzyme+EDTA (× ) are indicated in black. The initial rate of production formation is underestimated. (e) Plot of k_ss versus A_o. Data represent the mean±s.e.m. of three independent experiments.