Figure 5. Structural basis for slow product release.

(a) Electrostatic interactions of R³¹⁴ and K¹⁴⁸ with the 5′-P of the substrate strand. (b) Time-dependent nucleolytic reaction of hEXOG R314A_(monomer) (150 nM) on 3′-F dsDNA (200 nM). (c) Quantification of digestion. Data represent the mean±s.e.m. of three independent experiments.