Table 4.
Optimization of five nutrient variables (X1-X5) and two operating conditions (A, B) using Taugchi’s experimental design for the production of dry cell mass
| Runs order | Nutrient variables (g/l) and operating conditions levels a | Response Dry cell mass (g/l) | ||||||
|---|---|---|---|---|---|---|---|---|
| X1 | X 2 | X3 | X4 | X5 | A | B | ||
| 1 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 8.99 ± 0.02 |
| 2 | 3 | 1 | 3 | 4 | 2 | 2 | 1 | 9.12 ± 0.06 |
| 3 | 2 | 3 | 4 | 1 | 2 | 3 | 1 | 10.22 ± 0.04 |
| 4 | 4 | 1 | 4 | 2 | 3 | 4 | 1 | 13.47 ± 0.12 |
| 5 | 4 | 4 | 1 | 3 | 2 | 1 | 2 | 13.01 ± 0.01 |
| 6 | 3 | 2 | 2 | 3 | 1 | 2 | 2 | 8.21 ± 0.07 |
| 7 | 4 | 4 | 2 | 1 | 4 | 3 | 2 | 13.55 ± 0.01 |
| 8 | 2 | 1 | 3 | 3 | 4 | 4 | 2 | 11.32 ± 0.05 |
| 9 | 1 | 3 | 3 | 3 | 3 | 2 | 3 | 10.91 ± 0.05 |
| 10 | 2 | 2 | 4 | 4 | 3 | 2 | 3 | 11.21 ± 0.07 |
| 11 | 2 | 4 | 2 | 2 | 1 | 2 | 3 | 12.87 ± 0.06 |
| 12 | 1 | 4 | 2 | 4 | 4 | 2 | 3 | 12.71 ± 0.06 |
| 13 | 1 | 4 | 1 | 1 | 3 | 3 | 1 | 11.82 ± 0.02 |
| 14 | 4 | 3 | 1 | 4 | 3 | 3 | 3 | 13.88 ± 0.03 |
| 15 | 3 | 3 | 3 | 2 | 4 | 4 | 3 | 11.49 ± 0.08 |
| 16 | 1 | 1 | 2 | 1 | 1 | 3 | 4 | 8.21 ± 0.05 |
The bold data represent that experiemental run which shows maximum L. Plantarum biomass production
aX1, Glucose; X 2, Yeast extract; X3, Cheese Whey; X4, (NH4)2SO4; X5 corn steep liquor. Other composition included sodium acetate (5 g/l), MgSO4.7H2O (0.3 g/l), MnSO4.4H2O (0.04 g/l), Tween 80 (0.2 g/l)