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. 2017 May 4;48:28. doi: 10.1186/s13567-017-0433-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ROS production. ROS produced by MDMs (A) or PAMs (B) were measured by chemiluminescence assay at 24 and 48 h PI with PRRSV. Infection with H. parasuis was performed immediately prior to measurement. Data are presented as the percentage of CL levels in non-infected control. Boxplots indicate the median (middle line), 25th and 75th percentiles (boxes), and maximum and minimum (whiskers) of five independent experiments performed in culture triplicates. Significant differences between tested groups are denoted by different letters (a, b, c, d) (p < 0.05).