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. 2016 Oct 23;25(24):5353–5364. doi: 10.1093/hmg/ddw351

Figure 4.

Figure 4

Hierarchical recruitment of EMG1, NOP14/NOC4L and UTP14A into the nucleolus. (A–C) HeLa cells were transfected with non-target siRNAs (siNT) or those targeting the factors indicated to the left of each panel and the localisation of EMG1 (green), NSUN5 (red) and UTP14A or NOP14 (purple) was determined by immunofluorescence using antibodies against the endogenous proteins. Nuclear material was visualised by DAPI staining (blue). The scale bar indicates 20 μm. (D) HEK293 cell lines expressing siRNA-resistant, untagged EMG1 or EMG1D86G at endogenous levels were transfected with siRNAs against endogenous EMG1 and co-transfected with either non-target siRNAs (siNT) or siRNAs against NOP14. The localisation of EMG1 and EMG1D86G was determined by immunofluorescence using an anti-EMG1 antibody. Nuclear material was visualised by DAPI staining (blue). The scale bar indicates 20 μm.