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. 2016 Oct 23;25(24):5311–5320. doi: 10.1093/hmg/ddw345

Figure 2.

Figure 2.

Cryo-TEM analysis of wild-type retinoschisin. (A) Blue-native PAGE and native-blot analysis of retinoschisin. Marked are two distinct bands for the octamer and dimer of octamer complexes. (B) Reference-free class averages of the octamer and (C) dimer of octamers formed in solution. Box size = 24 nm. (D) Map of the C8 symmetrical octamer complex at 13.6Å resolution. (E) Comparison of the SAXS discoidin domain model to the assembled octamer, the positions of the discoidin domains are shown. (F) Map of the retinoschisin dimer of octamers at 9.3Å resolution.