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. 2016 Oct 23;25(24):5311–5320. doi: 10.1093/hmg/ddw345

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© The Author 2016. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Destabilising effect of the H207Q mutation. (A) Reference-free class averages of H207Q octamers under negative-stain conditions. Box size = 35 nm. (B) Native-PAGE analysis of H207Q shows two species representing the octamer and dimer of octamers. (C) Differential scanning fluorimetry T_m comparison of wild-type and H207Q monomers (n = 5). (D) Static Light Scattering analysis of wild-type and H207Q monomeric protein during a temperature ramp experiment (n = 3) showing T_agg (WT) of 46.2°C and T_agg (H207Q) of 43.5°C. (E) Differential scanning fluorimetry T_mcomparison of wild-type and H207Q octamers (n = 5).