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. 2016 Oct 23;25(24):5311–5320. doi: 10.1093/hmg/ddw345

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© The Author 2016. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Cryo-EM analysis of the R141H dimer of octamers. (A) Blue-native PAGE and native-blot analysis of R141H retinoschisin. (B) Reference-free class averages of R141H dimer of octamers. Box size = 33 nm (C) Three-dimensional reconstruction at 4.2Å resolution of the R141H dimer of octamers. Shown is the unmasked map. (D) Fitting of the R141H discoidin domain model (residues 63-219) shown in red, with associated subunits in the opposing octamer shown in blue. The central density of Rs1 domains has been removed for clarity. (E) Fit into single subunit showing the fitting of the β-sheets and positions of the spike regions. (F) Comparison between wild-type hexadecamer (blue, emd_6425) and R141H retinoschisin (red) structures at 5Å resolution.