Fig. 2.

Downregulating ANP32A improves spatial learning and memory in htau mice. a Schematics of the procedure. Lentivirus co-expressing RFP and ANP32A shRNA (siANP) or the scrambled control shRNA (siC) (2×10⁹ TU/ml) were stereotaxically injected into the hippocampal CA3 of 11 m-old htau mice (htau-siC and htau-siANP) or the age-matched wild-type littermates (wt-siC). One month later, learning and memory were detected in Morris water maze (MWM) and then the mice were sacrificed for the biochemical measurements. b The representative image confirming expression of the injected lentivirus in hippocampal CA3. c, d The knockdown efficiency of ANP32A protein in hippocampal CA3 measured by Western blotting and quantitative analysis. Data were presented as mean ± SD (two-way ANOVA followed by Bonferroni’ s post hoc test). e The knockdown efficiency of ANP32 mRNA in the hippocampal CA3 detected by RT-PCR. Data were presented as mean ± SD (two-way ANOVA followed by Bonferroni’ s post hoc test). f The knockdown efficiency of ANP32A protein in hippocampal CA3 measured by immunohistochemistry. g Arresting ANP32A overexpression improves spatial learning shown by the reduced escape latency in htau mice measured in MWM. Data were presented as mean ± s.e.m (two-way repeated measures ANOVA followed by Bonferroni’ s post hoc test). h The representative swimming traces of the mice recorded in the maze after removed the platform in 7th day. i–k Arresting ANP32A overexpression improves spatial memory shown by the reduced latency to reach the platform quadrant (i), the increased crossing times in the platform site (j) and time spent in the target quadrant (k) measured at day 7 by removed the platform (n = 11 each group). Data were presented as mean ± SD (two-way ANOVA followed by Bonferroni’ s post hoc test). l, m Arresting ANP32A overexpression did not change the distance travelled (l) and swimming speed (m) of the mice in water maze task (two-way ANOVA followed by Bonferroni’ s post hoc test). *, p<0.05; **, p<0.01 vs wt-siC; ^#, p<0.05; ^##, p<0.01 vs htau-siC