Fig. 3.

Downregulating ANP32A improves synaptic plasticity. a–c Arresting ANP32A overexpression in htau mice restores the slopes of fEPSP (a,b) with no influence on paired-pulse ratio (c) in hippocampal CA3 by stimulating mossy fibers. (n=7-8 slices from 4 mice for each group). d–f Arresting ANP32A overexpression in htau mice restores total, thin- and mushroom-shaped spines in the hippocampal CA3 neurons (at least 20 neurons from six to seven mice per group were analyzed by the Sholl). Bar=5 μm. Data were presented as mean ± SD. *, p<0.05, **, p<0.01 vs wt-siC; ^#, p<0.05; ^##, p<0.01 vs htau-siC (two-way ANOVA followed by Bonferroni’ s post hoc test)