Table 1.
ARE-bla Assay Results Summary.
| Measurea | log10 Ci, for i = a | Chemicalj, for j = b | P adj c | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | All | |||
| M | 1 | −3.00 | −0.750 | 1.67 | −1.18 | −3.15 | −0.230 | −1.73 | 0.080 | −1.91 | −1.13 | .67 |
| M | 2 | −3.57 | −0.730 | −3.56 | 1.29 | −1.54 | −0.970 | −4.07 | −3.57 | −1.76 | −2.08 | .67 |
| M | 3 | −4.60 | −3.52 | −0.68 | −3.57 | −5.86 | −1.52 | −2.28 | −5.66 | −5.08 | −3.61 | .24 |
| M | 4 | −2.58 | −2.06 | −3.00 | −1.97 | −1.00 | −2.92 | −5.75 | −2.95 | −3.89 | −2.90 | .67 |
| M | 5 | −4.17 | −1.50 | −4.84 | −3.43 | −3.72 | −2.65 | −2.71 | −5.45 | −3.54 | −3.56 | .67 |
| M | 6 | −2.74 | −3.25 | −2.55 | −2.45 | −2.04 | 0.740 | −2.70 | −2.30 | −3.36 | −2.29 | .64 |
| M | 7 | −4.62 | −2.68 | −2.62 | −0.630 | −1.98 | −1.83 | −1.86 | −3.60 | −1.88 | −2.41 | .67 |
| M | 8 | −3.61 | −1.33 | 0.130 | −3.21 | −2.17 | 5.99 | 3.99 | 1.58 | 2.46 | 0.510 | .0037 |
| M | 9 | −3.51 | −0.20 | −0.16 | −2.94 | −2.63 | 2.48 | 10.5 | 9.33 | 6.72 | 2.29 | .0013 |
| M | 10 | −3.44 | 0.720 | 7.53 | −1.41 | 11.0 | 10.5 | 20.0 | 37.6 | 37.3 | 13.3 | <10−10 |
| M | 11 | 9.10 | 14.4 | 26.8 | 4.85 | 47.3 | 13.1 | 41.8 | 69.4 | 78.3 | 33.9 | <10−4 |
| M | 12 | 38.1 | 39.4 | 43.1 | 25.9 | 71.2 | 28.5 | 71.1 | 82.0 | 83.8 | 51.5 | <10−9 |
| SD | 1 | 2.80 | 2.85 | 3.26 | 1.14 | 5.44 | 3.35 | 4.77 | 4.50 | 2.48 | 3.58 | .71 |
| SD | 2 | 2.14 | 0.910 | 1.30 | 4.29 | 3.57 | 6.80 | 4.27 | 2.73 | 2.71 | 3.72 | .29 |
| SD | 3 | 2.18 | 1.39 | 2.51 | 1.48 | 2.51 | 4.05 | 3.57 | 2.54 | 2.11 | 2.95 | .71 |
| SD | 4 | 1.97 | 1.75 | 2.32 | 1.44 | 2.05 | 4.95 | 1.36 | 2.57 | 1.79 | 2.57 | .71 |
| SD | 5 | 1.78 | 1.20 | 3.06 | 1.73 | 4.09 | 2.19 | 3.21 | 1.91 | 1.80 | 2.52 | .71 |
| SD | 6 | 2.79 | 5.40 | 3.90 | 2.80 | 2.85 | 3.28 | 0.36 | 0.890 | 2.15 | 3.00 | .041 |
| SD | 7 | 2.69 | 4.35 | 3.01 | 3.27 | 2.15 | 3.15 | 3.53 | 1.32 | 2.77 | 2.95 | .71 |
| SD | 8 | 3.96 | 3.87 | 1.52 | 2.36 | 2.85 | 5.77 | 3.66 | 1.59 | 1.76 | 4.37 | .64 |
| SD | 9 | 5.86 | 4.20 | 4.40 | 1.72 | 4.61 | 2.32 | 5.06 | 8.66 | 4.91 | 6.89 | .71 |
| SD | 10 | 14.3 | 4.18 | 3.52 | 1.65 | 7.60 | 4.19 | 4.31 | 8.56 | 8.18 | 16.1 | .088 |
| SD | 11 | 5.11 | 3.88 | 6.44 | 2.73 | 5.48 | 7.75 | 10.1 | 7.66 | 28.0 | 27.6 | <10−3 |
| SD | 12 | 8.84 | 16.0 | 8.63 | 6.15 | 8.74 | 11.4 | 8.59 | 4.80 | 3.54 | 23.2 | .71 |
| N | 1 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 45 | – |
| N | 2 | 5 | 4 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 44 | – |
| N | 3 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 4 | 44 | – |
| N | 4 | 5 | 5 | 5 | 5 | 5 | 5 | 5** | 5 | 5 | 45 | – |
| N | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 45 | – |
| N | 6 | 5 | 5 | 5 | 5 | 5 | 5 | 5** | 5 | 5 | 45 | – |
| N | 7 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 45 | – |
| N | 8 | 5 | 5 | 5 | 4 | 5 | 5 | 5 | 5 | 5 | 44 | – |
| N | 9 | 5 | 5 | 5 | 4 | 5 | 5 | 5 | 5 | 5 | 44 | – |
| N | 10 | 5 | 5 | 5 | 5 | 5 | 5 | 5** | 5** | 5** | 45 | – |
| N | 11 | 5* | 5** | 5** | 5* | 5** | 5* | 5** | 5** | 5* | 45 | – |
| N | 12 | 4** | 5** | 5** | 5** | 5** | 5** | 4** | 3** | 4** | 40 | – |
Abbreviation: ANOVA, analysis of variance; ARE, antioxidant response element; PA, percentage activity.
aMeasures: M = arithmetic mean of chemical-specific net activities (PAs), SD = standard deviation of M, N = number of measured PAs/chemical at each concentration. M and SD values are shown rounded to 3 significant digits. N values shown with 1 or 2 asterisks correspond to sets of activities that differ significantly from zero by 2-tailed t test. Values of log10(concentration Ci, in Molar units), i = {1,…,12}, are {−9.66, −9.19, −8.71, −8.23, −7.76, −7.28, −6.8, −6.33, −5.85, −5.37, −4.89, −4.42}.
bChemicals i = {1,…,9} are {alachlor, 2-amino-4-methylbenzothiazole, 2-amino-6-nitrobenzothiazole, 3-dimethylaminophenol, N,N-dimethyl-p-nitrosoaniline, N,N-dimethyl-p-phenylenediamine, 8-hydroxyquinoline, melphalan, and 1,10-phenanthroline monohydrate}.
cListed values of P adj each represent corresponding Hommel Bonferroni-type adjusted P values from 12 independent 1-way ANOVA (or for j = 6 and 11, Kruskal-Wallis) tests of means (M) homogeneity and from 12 independent Bartlett tests of variance (Sqrt[SD]) homogeneity. The indicated total of N chemical-specific PA measures made were each approximately normally distributed (P adj > .05 for each of 9 chemicals) at each concentration, except for chemical 5 measured at the highest concentration (P adj = .0024).