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Effect of H-SN1 on LPS-induced nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling activation in lung tissues. The phosphorylation of NF-κBp65 and extracellular-signal related kinase ½ (ERK1/2) proteins were analyzed by Western blot. (A) A representative immunoblot from three separate experiments with similar results is shown. (B–D) Relative protein levels were quantified by ImageJ software and expressed as optical density ratio. ∗p < 0.05 and ∗∗p < 0.01.
