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. 2017 May 5;8:246. doi: 10.3389/fphar.2017.00246

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Copyright © 2017 Wu, Wang, Luo, Li, Tian, Jiang, Zheng, Zhu, Lu and Xia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of H-SN1 on LPS-induced cytokines mRNA expression and production in RAW264.7 cells. The mRNA expression of TNF-α (A), IL-6 (B), and IL-1β (C) was analyzed at 3 and 6 h post stimulation by RT-PCR. The production of TNF-α (D), IL-6 (E), and IL-1β (F) in supernatants was measured at 3 and 6 h post stimulation by enzyme-linked immunosorbent assay (ELISA). Data were presented as mean ± SEM (n = 4 in each group), ^∗∗p < 0.01 and ^&&p < 0.01 vs. Control group, ^#p < 0.05, ^##p < 0.01, ⁺p < 0.05, and ⁺⁺p < 0.01 vs. LPS group.