Table 1.
Effect of incubation of fibroblast cultures and sonicates with EcV (0.5 µg ml–1 MEM for 4 h) on activities of antioxidant enzymes.
| Samples assayed (n = 12) | Enzymatic parameters (µmole min–1 mg–1 protein) |
|||
|---|---|---|---|---|
| GPx | CAT | SOD | GR | |
| Control cultures | 1.93 ± 0.16 | 3.58 ± 0.29 | 26.1 ± 2.29 | 2.48 ± 0.23 |
| Cultures + EcV | 1.14 ± 0.11* | 2.22 ± 0.20* | 17.1 ± 1.48* | 1.36 ± 0.13* |
| Control sonicates | 1.88 ± 0.15 | 3.46 ± 0.30 | 24.8 ± 2.10 | 2.53 ± 0.22 |
| Sonicates + EcV | 1.91 ± 0.16 | 3.41 ± 0.28 | 25.6 ± 2.31 | 2.46 ± 0.23 |
Confluent passage 5 cultures were used. The values shown are means ± SD for triplicates of the 12 cultures and sonicates. EcV = Echis coloratus crude venom, GPx = glutathione peroxidase, CAT = catalase, SOD = superoxide dismutase, GR = glutathione reductase. *Significant at p < 0.001 upon comparison of the values obtained for all assayed parameters in EcV-incubated cultures against those recorded for venom-free controls.