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. Author manuscript; available in PMC: 2018 Apr 15.
Published in final edited form as: Cancer Res. 2017 Feb 16;77(8):2102–2111. doi: 10.1158/0008-5472.CAN-16-2548

Fig. 4. Influence of OCTN1 on cytarabine uptake in AML cells.

Fig. 4

Gene expression of OCTN1 and ENT1 (two probe sets) in primary AML blast samples from pediatric patients used in the survival analysis shown in Fig. 1A–B, as determined by microarray analysis (A). Each column represents an individual primary sample, and columns are categorized by cytogenetic AML subtypes of prognostic relevance. (B) Protein expression of OCTN1 in a panel of 12 AML cell lines. (C) Cellular uptake of cytarabine (Ara-C; 1 μM; 5 min uptake) with or without NBMPR pre-incubation was measured in OCI-AML3 cells 48 hours after transfection with a non-targeting (NT) control siRNA or a siRNA targeting OCTN1. Results are shown as cytarabine uptake as compared to cells transfected with NT siRNA. Data are representative of two independent experiments done in triplicate. OCTN1 protein expression was determined by western blot from membrane extraction of OCI-AML3 cells 48 hours post-transfection, and the transferrin receptor served as loading control. The relative expression difference of OCTN1 after RNAi is indicated by the numbers above the lanes.