Figure 2.

Resolving N-glycosylation and other types of modification increasing mAb micro-heterogeneity on therapeutic mAbs. (A) Deconvoluted native MS spectra of N-glycosylated and deglycosylated trastuzumab, revealing only minor modifications besides N-glycosylation. (B) Deconvoluted native MS spectra of infliximab revealing incomplete C-terminal lysine processing of this mAb. The annotations of the glycoforms are color-coded according to the number of clipped Lysine residues. (C) Deconvoluted native MS spectra of bevacizumab suggesting 1–4 hexose residues are still attached after N-deglycosylation revealing the occurrence of extensive glycation (annotated with the green dots).