Table 2.
Average N-glycan Profiles from L-Galactose Supplemented Cultures.
L-Gal Conc. (mM) | Man5 (%) | Man6 (%) | G0G*- GlcNAc (%) | G0F- GlcNAc (%) | G0(%) | G0G*(%) | G0F (%) | G1G* (%) | G1F (%) | Fucose Reduction (%) |
---|---|---|---|---|---|---|---|---|---|---|
0 | 1.3 | 0 | 0 | 5.6 | 0.4 | 0 | 83.3 | 0 | 6.7 | 1.7 |
0.1 | 1.9 | 0 | 0 | 6.0 | 0.5 | 13.5 | 70.6 | 1.3 | 5.1 | 17.2 |
1 | 2.0 | 0.9 | 4.1 | 4.0 | 0.5 | 37.0 | 43.6 | 2.5 | 3.6 | 47.4 |
5 | 2.4 | 0 | 7.8 | 0.7 | 0 | 72.9 | 6.5 | 5.1 | 1.2 | 91 |
10 | 2.5 | 0.4 | 8.5 | 0 | 0 | 80.5 | 0 | 5.7 | 0 | 100 |
Note: G* represents galactose in the L-fucose position on the N-glycan.