Figure 7.

Schematic overview of the fate of TmTNF-bound anti-TNF. Binding of anti-TNF to cell surface expressed TmTNF (1) results in transducing intracellular ‘Reverse’ signal (2) and formation of a clathrin-coated pit (3) resulting in endocytosis of the complex into the endosomes (4) followed by delivery of the complex to the phagolysosome compartment (5) where proteolytically processed anti-TNF peptides are loaded onto HLA-DR and subsequently displayed as HLA-DR-associated peptides on the cell surface (6).